Mouse IL12A & IL12B (IL12A & IL12B Heterodimer) recombinant protein

Mouse IL12A & IL12B (IL12A & IL12B Heterodimer) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

P43431

Human Cells

A DNA sequence encoding the p35 subunit of mouse IL12, termed as IL12A (P43431) (Met 1-Ala 215) was fused with the Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the p40 subunit of mouse IL12, termed as IL12B (P43432) (Met 1-Ser 335) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified.

Mouse

The recombinant mouse IL12 heterodimer of IL12A/IL12B, IL12A is Fc chimera, comprises 758 (434 +324) amino acids and has a calculated molecular mass of 85.9 (48.7 + 37.2) kDa. The apparent molecular mass of rh IL12 heterodimer is approximately 55 & 45 kDa respectively in SDS-PAGE under reducing conditions.

> 85 % as determined by SDS-PAGE

1. Measured by its binding ability in a functional ELISA. Immobilized mouse IL12RB2-His at 10 μg/ml (100 μl/well) can bind Mouse IL12A & IL12B Heterodimer Protein (Cat:CT024-M0208H). The EC₅₀ of Mouse IL12A & IL12B Heterodimer Protein (Cat:CT024-M0208H) is 50.2-117.2 ng/ml.
2. Measured in a cell proliferation assay using Anti-CD3-stimulated PBMC. The ED₅₀ for this effect is typically 0.2-1 ng/mL.