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Product Name
Human IL12A & IL12B (IL12A & IL12B Heterodimer) recombinant protein
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Protein name
Interleukin-12 subunit alpha
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Uniprot ID
P29459
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Gene Name
IL12A; NKSF1
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Source/Expression Host
Human Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the p35 subunit of human IL-12, termed as IL12A (NP_000873.2) (Met 1-Ser 219) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1 ; A DNA sequence encoding the p40 subunit of human IL-12, termed as IL12B (NP_002178.2) (Met 1-Ser 328) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified.
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Protein Species
Human
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Molecular weight
The recombinant IL12 heterodimer of human IL12A/IL12B comprises 525 (208 + 317) amino acids and has a calculated molecular mass of 60.2 (24 + 36.2) kDa. The apparent molecular mass of rh IL12 heterodimer is approximately 40 & 43 kDa respectively in SDS-PAGE under reducing conditions.
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Purity
> 95 % as determined by SDS-PAGE
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Activity
1. Measured by its ability to bind biotinylated recombinant human IL12RB1 in a functional ELISA.
2. Measured in a cell proliferation assay using Anti-CD28 and Anti-CD3-stimulated PBMC.
The ED50 for this effect is typically 0.1-0.6 ng/mL. -
Validations
Human IL-12 (IL12A & IL12B Heterodimer) Protein SDS-PAGE
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