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Product Name
Human IL12A & IL12B recombinant protein (C-His)
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Protein short names
CLMF,CLMF2,IL-12A,IL-12B,IL12,IMD28,IMD29,NFSK,NKSF,NKSF1,NKSF2,P35
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Uniprot ID
P29459 & P29460
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Source/Expression Host
Human Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the p35 subunit of human IL-12, termed as IL12A (NP_000873.2) (Met1-Ser219) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1 ; A DNA sequence encoding the p40 subunit of human IL-12, termed as IL12B (NP_002178.2) (Met1-Ser328) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified.
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Protein Species
Human
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Molecular weight
The recombinant IL12 heterodimer of human IL12A/IL12B comprises 525 (208 + 317) amino acids and has a calculated molecular mass of 60.2 (24 + 36.2) kDa.
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Purity
> 95 % as determined by SDS-PAGE
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Validations
Human IL12/IL12A & IL12B Protein 15483
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