Mouse ICAM-2/CD102 recombinant protein

Mouse ICAM-2/CD102 recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Intercellular adhesion molecule 2 (ICAM-2, CD102), belongs to the ICAM family consisting of three members identified as ligands for integrin receptors. It is a type I transmembrane glycoprotein with two Ig-like C2-type domains, and binds to the leukocyte integrins LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). As a second ligand of leukocyte function-associated antigen-1, ICAM-2 functions as a costimulatory molecule for effector cells. ICAM-2 is mainly expressed on vascular endothelial and hematopoietic cells. Interactions of ICAM-2 and the integrin receptors mediate cell adhesion in a wide range of lymphocyte, monocyte, natural killer cell, and granulocytewith other cells, and play important roles in many adhesion-dependent immune and inflammation responses, such as T cell aggregation, NK-cell cytotoxicity and migration, lymphocyte recirculation, etc. Serum levels of ICAM-2 correlated significantly with the inflammatory and course sequences of trichinosis in mice and had a similar relation with blood eosinophilia. So, estimation of ICAM-2 serum levels may prove useful in diagnosis of trichinosis recent infections, and in monitoring the prognosis and response to treatment.

Intercellular adhesion molecule 2

ICAM2; RP23-418O11.10-001; ICAM-2; LY-60; CD102

P35330

Icam2; Icam-2

Human Cells

A DNA sequence encoding the mouse ICAM2 (NP_034624.1) (Met 1-Gln 222)was expressed with six amino acids (LEVLFQ) at the C-terminus.

Mouse

The recombinant mouse ICAM2 consists of 210 amino acids and has a calculated molecular mass of 23.6 kDa. The recombinant protein migrates as an approximately 38-42 kDa band in SDS-PAGE under reducing conditions.

> 85 % as determined by SDS-PAGE

Measured by the ability of the immobilized protein to support the adhesion of PMA stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells.
When 5 x 10E4 cells/well are added to rmICAM1 coated plates (12.5 μg/mL with 100 μL/well), > 30% cells will adhere after 1 hour incubation at 37℃.