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Product Name
Human ICAM-2/CD102 (His & Fc Tag) recombinant protein
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Description
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM2 may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance.
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Protein name
Intercellular adhesion molecule 2
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Protein short names
ICAM-2; RP23-418O11.10-001; ICAM2; CD102; LY-60
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Uniprot ID
Q6FHE2
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Gene Name
ICAM2
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Source/Expression Host
Human Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the extracellular domain (Met 1-Gln 223) of human ICAM2 (NP_000864.2) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
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Protein Species
Human
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Molecular weight
The recombinant human ICAM2/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 449 amino acids and has a predicted molecular mass of 50.3 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhICAM2/Fc monomer is approximately 85-95 kDa due to glycosylation.
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Purity
> 97 % as determined by SDS-PAGE
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Activity
Measured by the ability of the immobilized protein to support the adhesion of PMA stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells.
When cells are added to ICAM2 coated plates (12.5 μg/mL, 100 μL/well), approximately 50 % - 60 % will adhere specifically. -
Validations
Human ICAM-2 / CD102 Protein (His & Fc Tag) SDS-PAGE
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