Human ICAM-2/CD102 (His Tag) recombinant protein

Human ICAM-2/CD102 (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Intercellular adhesion molecule 2 (ICAM-2, CD102), belongs to the ICAM family consisting of three members identified as ligands for integrin receptors. It is a type I transmembrane glycoprotein with two Ig-like C2-type domains, and binds to the leukocyte integrins LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). As a second ligand of leukocyte function-associated antigen-1, ICAM-2 functions as a costimulatory molecule for effector cells. ICAM-2 is mainly expressed on vascular endothelial and hematopoietic cells. Interactions of ICAM-2 and the integrin receptors mediate cell adhesion in a wide range of lymphocyte, monocyte, natural killer cell, and granulocytewith other cells, and play important roles in many adhesion-dependent immune and inflammation responses, such as T cell aggregation, NK-cell cytotoxicity and migration, lymphocyte recirculation, etc. Serum levels of ICAM-2 correlated significantly with the inflammatory and course sequences of trichinosis in mice and had a similar relation with blood eosinophilia. So, estimation of ICAM-2 serum levels may prove useful in diagnosis of trichinosis recent infections, and in monitoring the prognosis and response to treatment.

Intercellular adhesion molecule 2

CD102; LY-60; ICAM-2; RP23-418O11.10-001; ICAM2

Q6FHE2

ICAM2

Human Cells

A DNA sequence encoding the extracellular domain (Met 1-Gln 223) of human ICAM2 (NP_000864.2) was fused with a polyhistidine tag at the C-terminus.

Human

The recombinant human ICAM2 consists of 213 amino acids after removal of the signal peptide and has a predicted molecular mass of 24 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhICAM2 is approximately 50-55 kDa due to glycosylation.

> 97 % as determined by SDS-PAGE

Measured by the ability of the immobilized protein to support the adhesion of PMA stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When cells are added to ICAM2 coated plates (12.5 μg/ml, 100 μl/well), approximately 35 %-45% will adhere specifically.