Mouse Coagulation Factor VII (His Tag) recombinant protein

Cat.#: 503964

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Product Information

  • Product Name
    Mouse Coagulation Factor VII (His Tag) recombinant protein
  • Documents
  • Description
    Coagulation factor VII, also known as Serum prothrombin conversion accelerator, Factor VII, F7 and FVII, is a member of the peptidase S1 family. Factor VII is one of the central proteins in the coagulation cascade. It is an enzyme of the serine protease class, and Factor VII (FVII) deficiency is the most frequent among rare congenital bleeding disorders. Factor VII contains two EGF-like domains, one Gla (gamma-carboxy-glutamate) domain and one peptidase S1 domain. The main role of factor VII is to initiate the process of coagulation in conjunction with tissue factor (TF). Tissue factor is found on the outside of blood vessels, normally not exposed to the blood stream. The action of the Factor VII is impeded by tissue factor pathway inhibitor (TFPI), which is released almost immediately after initiation of coagulation. Factor VII is vitamin K dependent and is produced in the liver. Upon vessel injury, tissue factor is exposed to the blood and circulating Factor VII. Once bound to TF, FVII is activated to FVIIa by different proteases, among which are thrombin (factor IIa), factor Xa, IXa, XIIa, and the FVIIa-TF complex itself. Recombinant activated factor VII (rFVIIa) is a haemostatic agent, which was originally developed for the treatment of haemophilia patients with inhibitors against factor FVIII or FIX. FVIIa binds specifically to endothelial protein C receptor (EPCR), a known cellular receptor for protein C and activated protein C, on the endothelium. rFVIIa is a novel hemostatic agent, originally developed for the treatment of hemorrhage in hemophiliacs with inhibitors, which has been successfully used recently in an increasing number of nonhemophilic bleeding conditions.
  • Protein name
    Coagulation factor VII
  • Protein short names
    F7; SPCA; CF7; AI132620; MFVII; COAGULATION FACTOR VII; FVII
  • Uniprot ID
    Q542C2
  • Gene Name
    F7; Cf7
  • Source/Expression Host
    CHO Cells
  • Expression Plasmid/cDNA
    A DNA sequence encoding the mouse FVII (NP_034302.2) (Met 1-Leu 446) was fused with the a polyhistidine tag at the C-terminus.
  • Protein Species
    Mouse
  • Molecular weight
    The mature form of mouse FVII consists of 416 amino acids after removal of the signal peptide and the propeptid, and has a predicted molecular mass of 47 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm FVII is approximately 55-50 kDa due to glycosylation.
  • Purity
    > 98 % as determined by SDS-PAGE
  • Activity
    Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC (R&D Systems, Catalog # ES011). The specific activity is >4 pmoles/min/μg.
  • Validations

    Mouse Coagulation Factor VII / FVII / F7 Protein (His Tag) SDS-PAGE

    Mouse Coagulation Factor VII / FVII / F7 Protein (His Tag) SDS-PAGE

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"