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Product Name
Mouse Coagulation Factor VII (His Tag) recombinant protein
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Description
Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa will also convert factor IX to factor IXa in the presence of tissue factor and calcium (By similarity).
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Protein name
Coagulation factor VII
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Protein short names
F7; CF7; AI132620; SPCA; COAGULATION FACTOR VII; MFVII; FVII
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Uniprot ID
Q542C2
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Gene Name
F7; Cf7
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Source/Expression Host
CHO Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the mouse F? (NP_034302.2) (Met 1-Leu 446) was fused with the a polyhistidine tag at the C-terminus.
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Protein Species
Mouse
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Molecular weight
The mature form of mouse F? consists of 416 amino acids after removal of the signal peptide and the propeptid, and has a predicted molecular mass of 47 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm F? is approximately 56-63 kDa due to glycosylation.
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Purity
> 90 % as determined by SDS-PAGE
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Activity
Measured by its binding ability in a functional ELISA.
Immobilized mouse F7-his at 10 μg/ml (100 μl/well) can bind biotinylated mouse F3-his (Cat：50413-M08H). The EC50 of biotinylated mouse F3-his (Cat：50413-M08H) is 0.1-0.3 μg/ml. -
Validations
Mouse Coagulation Factor VII / FVII / F7 Protein (His Tag) SDS-PAGE
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