Mouse CD180/RP105 (His Tag) recombinant protein

Mouse CD180/RP105 (His Tag) recombinant protein

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The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD180, also known as RP105, is a B-cell surface molecule belonging to the family of pathogen receptors, Toll-like receptors (TLR). CD180 has an extracellular leucine-rich repeats and a short cytoplasmic tail. CD180 / RP105 interact with an extracellular molecule named MD1 and then together form the cell surface receptor complex RP105 / MD1 which induces B-cell activation in humans and mice, leading to proliferation and up-regulation of a costimulatory molecule, B7.2 / CD86. CD180 / RP105 also has a role in LPS response because B cells lacking RP105 show hyporesponsiveness to LPS.

CD180 antigen

F630107B15; MGC126234; MGC126233; LY64; CD180; RP105; LY78

Q62192

Cd180; Ly78; Rp105

Human Cells

A DNA sequence encoding the mouse CD180 (NP_032559.2) extracellular domain (Met 1-Ser 626) was expressed, fused with a polyhistidine tag at the C-terminus.

Mouse

The recombinant mouse CD180 consists of 617 amino acids and predicts a molecular mass of 69.4 kDa. As a result of glycosylation, the apparent molecular mass of rm CD180 is approximately 80 kDa in SDS-PAGE under reducing conditions.

> 92 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA. Immobilized mouse CD180 at 2 μg/ml (100 μl/well) can bind biotinylated mouse MD-1 with a linear ranger of 6.25-50 μg/ml.