MonoMethyl-Histone H4-R3 pAb

Cat.#: 164047

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Product Information

  • Product Name
    MonoMethyl-Histone H4-R3 pAb
  • Documents
  • Description
    Polyclonal antibody to MonoMethyl-Histone H4-R3
  • Tested applications
    WB, IHC, IF, IP
  • Species reactivity
    Human, Mouse, Rat, Other (Wide Range)
  • Alternative names
    HIST2H4A antibody; FO108 antibody; H4 antibody; H4/n antibody; H4F2 antibody; H4FN antibody; HIST2H4 antibody; histone H4 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: A synthetic peptide of human MonoMethyl-Histone H4-R3
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
    IHC 1:50 - 1:200
    IF 1:50 - 1:200
    IP 1:50 - 1:200
  • Validations

    Western blot - MonoMethyl-Histone H4-R3 pAb

    Western blot - MonoMethyl-Histone H4-R3 pAb

    Western blot analysis of extracts of various cell lines, using MonoMethyl-Histone H4-R3 antibody .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.

    Immunofluorescence - MonoMethyl-Histone H4-R3 pAb

    Immunofluorescence - MonoMethyl-Histone H4-R3 pAb

    Immunofluorescence analysis of 293T cells using MonoMethyl-Histone H4-R3 antibody . Blue: DAPI for nuclear staining.

  • Background
    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"