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Product Name
METTL1 antibody
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Description
METTL1 Rabbit Polyclonal antibody. Positive IHC detected in human gliomas. Positive WB detected in A2780 cells, human brain tissue, human heart tissue, human liver tissue. Observed molecular weight by Western-blot: 31kd
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Tested applications
ELISA, WB, IHC
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Species reactivity
human, mouse, rat; other species not tested.
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Alternative names
METTL1 antibody; TRM8 antibody; FLJ95748 antibody; C12orf1 antibody; tRNA(m7G46) methyltransferase antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of METTL1 recombinant protein (Accession Number: NM_005371). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IHC: 1:20-1:200
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Validations
A2780 cells were subjected to SDS PAGE followed by western blot with Catalog No:112516(METTL1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:112516(METTL1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:112516(METTL1 antibody) at dilution of 1:100 (under 40x lens)
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Background
METTL1 represents methyltransferase like 1
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References
- Okamoto M, Fujiwara M, Hori M. tRNA modifying enzymes, NSUN2 and METTL1, determine sensitivity to 5-fluorouracil in HeLa cells. PLoS genetics. 10(9):e1004639. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"