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Product Name
Anti-METTL1 antibody
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Description
Rabbit polyclonal to METTL1
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Tested applications
ELISA, WB, IHC-P, IP
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Species reactivity
Human METTL1
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Alternative names
TRM8 antibody; TRMT8 antibody; C12orf1 antibody; YDL201w antibody; 2810012D02Rik antibody; 2810012D02Rik antibody; C12orf1 antibody; FLJ95748 antibody; Mettl1 antibody; METTL1 antibody; TRM8 antibody; YDL201w antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Human METTL1 (rh METTL1; NP_005362.3; Asp 32-Gln 265). METTL1 specific IgG was purified by Human METTL1 affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 10-20 μg/mL
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human METTL1. The detection limit for Human METTL1 is approximately 0.00245 ng/well.
IHC-P: 0.1-2 μg/mL
IP: 1-4 μg/mg of lysate
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Validations
METTL1 Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
METTL1 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunoprecipitation
METTL1 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of human METTL1 in human bladder carcinoma with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections). The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).
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Background
tRNA (guanine-N(7)-)-methyltransferase, also known as Methyltransferase-like protein 1, tRNA (m7G46)-methyltransferase and METTL1, is a nucleus protein which belongs to the methyltransferase superfamily and TrmB family. METTL1 gene, has been identified by its sequence similarity to the yeast ORF YDL201w. The human cDNA and the genomic structure of METTL1 have been analyzed. The transcript contains 1292 nucleotides and codes for a protein of 276 amino acids. The METTL1 gene product shows high sequence similarities to putative proteins from mouse, Drosophila melanogaster, Arabidopsis thaliana, Caenorhabditis elegans, and yeast (39.8% identity between all six species). Computer analyses of the deduced protein sequence reveal two highly conserved amino acid motifs, one of which is typical for methyltransferases. Both motifs are also present in hypothetical proteins from eubacteria. Disruption of the homologous yeast ORF YDL201w shows that the gene is at least not essential for vegetative growth in Saccharomyces cerevisiae.
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References
- Bahr, A.et al., 1999, Genomics. 57 (3):424-8.
- Wikman, H. et al., 2005,Genes Chromosomes Cancer. 42 (2):193-9.
- Cartlidge, RA. et al., 2005, EMBO J. 24 (9):1696-705.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"