Anti-METTL1 antibody

Anti-METTL1 antibody

• DataSheet
• Material Safety Data Sheets (MSDS)

Mouse monoclonal to METTL1

ELISA, WB, IP

Human METTL1
No cross-reactivity in ELISA with E.coli cell lysate

TRM8 antibody; TRMT8 antibody; C12orf1 antibody; YDL201w antibody; 2810012D02Rik antibody; 2810012D02Rik antibody; C12orf1 antibody; FLJ95748 antibody; Mettl1 antibody; METTL1 antibody; TRM8 antibody; YDL201w antibody

Mouse IgG1

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human METTL1 (rh METTL1; NP_005362.3; Asp 32-Gln 265). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Monoclonal

0.2 μm filtered solution in PBS with 5% trehalose

This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

tRNA (guanine-N(7)-)-methyltransferase, also known as Methyltransferase-like protein 1, tRNA (m7G46)-methyltransferase and METTL1, is a nucleus protein which belongs to the methyltransferase superfamily and TrmB family. METTL1 gene, has been identified by its sequence similarity to the yeast ORF YDL201w. The human cDNA and the genomic structure of METTL1 have been analyzed. The transcript contains 1292 nucleotides and codes for a protein of 276 amino acids. The METTL1 gene product shows high sequence similarities to putative proteins from mouse, Drosophila melanogaster, Arabidopsis thaliana, Caenorhabditis elegans, and yeast (39.8% identity between all six species). Computer analyses of the deduced protein sequence reveal two highly conserved amino acid motifs, one of which is typical for methyltransferases. Both motifs are also present in hypothetical proteins from eubacteria. Disruption of the homologous yeast ORF YDL201w shows that the gene is at least not essential for vegetative growth in Saccharomyces cerevisiae.

• Bahr, A.et al., 1999, Genomics. 57 (3):424-8.
• Wikman, H. et al., 2005,Genes Chromosomes Cancer. 42 (2):193-9.
• Cartlidge, RA. et al., 2005, EMBO J. 24 (9):1696-705.