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Product Name
LYN antibody
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Description
LYN Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, HL-60 cells, Jurkat cells, K-562 cells, L02 cells. Positive IP detected in HeLa cells. Positive IF detected in HeLa cells. Positive IHC detected in human kidney tissue, human hepat℃irrhosis tissue. Positive FC detected in Jurkat cells. Observed molecular weight by Western-blot: 53-56 kDa,66-70 kDa
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Tested applications
ELISA, WB, IHC, FC, IF, IP
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Species reactivity
Human; other species not tested.
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Alternative names
FLJ26625 antibody; JTK8 antibody; LYN antibody; Tyrosine protein kinase Lyn antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of LYN recombinant protein (Accession Number: NM_002350). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:112407(LYN antibody) at dilution of 1:1000
IP Result of anti-LYN (IP:Catalog No:112407, 4ug; Detection:Catalog No:112407 1:1000) with HeLa cells lysate 1200ug.
Immunohistochemistry of paraffin-embedded human kidney tissue slide using Catalog No:112407(LYN Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human kidney tissue slide using Catalog No:112407(LYN Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HeLa cells using Catalog No:112407(LYN Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
1X10^6 Jurkat cells were stained with 0.2ug LYN antibody (Catalog No:112407, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
LYN, also named as JTK8, p53Lyn and p56Lyn, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It is a non-receptor tyrosine-protein kinase that transmits signals from cell surface receptors and plays an important role in the regulation of innate and adaptive immune responses, hematopoiesis, responses to growth factors and cytokines, integrin signaling, but also responses to DNA damage and genotoxic agents. LYN is a component of the mTOR (mammalian target of rapamycin) pathway.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"