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Product Name
LONP1 antibody
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Description
LONP1 Mouse Monoclonal antibody. Positive WB detected in human liver tissue, human heart tissue. Positive IHC detected in human liver tissue, human heart tissue, human lung cancer tissue. Positive IF detected in C6 cells. Observed molecular weight by Western-blot: 100kD
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
hLON antibody; LON antibody; lon peptidase 1 antibody; mitOChondrial antibody; Lon protease like protein antibody; LonHS antibody; LONP antibody; LONP1 antibody; PIM1 antibody; PRSS15 antibody; Serine protease 15 antibody
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Isotype
Mouse IgG1
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Preparation
This antibody was obtained by immunization of LONP1 recombinant protein (Accession Number: NM_004793). Purification method: Protein G purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
human liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:107407(LONP1 antibody) at dilution of 1:500
Immunohistochemistry of paraffin-embedded human liver tissue slide using Catalog No:107407(LONP1 Antibody) at dilution of 1:50(under 10x lens)
Immunohistochemistry of paraffin-embedded human liver tissue slide using Catalog No:107407(LONP1 Antibody) at dilution of 1:50(under 40x lens)
Immunofluorescent analysis of C6 cells using Catalog No:107407(LONP1 Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG
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Background
LONP1(Lon protease homolog, mitochondrial) is also named as LONP, LONHS, HLON, LON, PRSS15, PIM1, MGC1498 and belongs to the peptidase S16 family. It seems to play a major role in the elimination of oxidatively modified proteins in the mitochondrial matrix(PMID:18021745). LONP1, also a nuclearly encoded and mitochondrially located stress-responsive protease, is involved in heme-mediated ALAS-1 turnover(PMID:21659532). It recognizes specific surface determinants or folds, initiates proteolysis at solvent-accessible sites, and generates unfolded polypeptides that are then processively degraded(PMID:15870080).
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