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Product Name
LONP1 antibody
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Description
LONP1 Rabbit Polyclonal antibody. Positive IP detected in mouse heart tissue. Positive WB detected in HeLa cells, HepG2 cells, human heart tissue, mouse heart tissue, rat heart tissue, rat lung tissue. Positive IF detected in HepG2 cells. Positive IHC detected in human lung cancer tissue. Observed molecular weight by Western-blot: 100 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
hLON antibody; LON antibody; lon peptidase 1 antibody; mitOChondrial antibody; Lon protease like protein antibody; LonHS antibody; LONP antibody; LONP1 antibody; PIM1 antibody; PRSS15 antibody; Serine protease 15 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of LONP1 recombinant protein (Accession Number: NM_004793). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:112295(LONP1 antibody) at dilution of 1:500
IP Result of anti-LONP1 (IP:Catalog No:112295, 3ug; Detection:Catalog No:112295 1:1000) with mouse heart tissue lysate 9500ug.
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:112295(LONP1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:112295(LONP1 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:112295(LONP1 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
LONP1(Lon protease homolog, mitochondrial) is also named as LONP, LONHS, HLON, LON, PRSS15, PIM1, MGC1498 and belongs to the peptidase S16 family. It seems to play a major role in the elimination of oxidatively modified proteins in the mitochondrial matrix(PMID:18021745). LONP1, also a nuclearly encoded and mitochondrially located stress-responsive protease, is involved in heme-mediated ALAS-1 turnover(PMID:21659532). It recognizes specific surface determinants or folds, initiates proteolysis at solvent-accessible sites, and generates unfolded polypeptides that are then processively degraded(PMID:15870080).
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References
- Kil IS, Ryu KW, Lee SK. Circadian Oscillation of Sulfiredoxin in the Mitochondria. Molecular cell. 59(4):651-63. 2015.
- Zheng J, Bizzozero OA. Decreased activity of the 20S proteasome in the brain white matter and gray matter of patients with multiple sclerosis. Journal of neurochemistry. 117(1):143-53. 2011.
- Moore F, Santin I, Nogueira TC. The transcription factor C/EBP delta has anti-apoptotic and anti-inflammatory roles in pancreatic beta cells. PloS one. 7(2):e31062. 2012.
- Cnop M, Abdulkarim B, Bottu G. RNA sequencing identifies dysregulation of the human pancreatic islet transcriptome by the saturated fatty acid palmitate. Diabetes. 63(6):1978-93. 2014.
- Shen XL, Zhang B, Liang R. Central role of Nix in the autophagic response to ochratoxin A. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 69:202-9. 2014.
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