Rat CD47 (His Tag) recombinant protein

Rat CD47 (His Tag) recombinant protein

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Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection (By similarity). Plays an important role in memory formation and synaptic plasticity in the hippocampus.

Leukocyte surface antigen CD47

ITGP; AW108519; CD47 ANTIGEN (RH-RELATED ANTIGEN; ANTIGEN IDENTIFIED BY MONOCLONAL ANTIBODY 1D8; AI848868; LEUKOCYTE SURFACE ANTIGEN CD47; IAP; INTEGRIN ASSOCIATED PROTEIN; RH-RELATED ANTIGEN; B430305P08RIK; OA3; INTEGRIN-ASSOCIATED PROTEIN; MER6; INTEGRIN-ASSOCIATED SIGNAL TRANSDUCER); CD47 GLYCOPROTEIN; 9130415E20RIK; INTEGRIN-ASSOCIATED SIGNAL TRANSDUCER; ANTIGENIC SURFACE DETERMINANT PROTEIN OA3; AA407862

P97829

Cd47

Human Cells

A DNA sequence encoding the rat CD47 (P97829-1) (Met1-Lys140) was expressed, fused with a polyhistidine tag at the C-terminus.

Rat

The recombinant rat CD47 comprises 133 amino acids and predicts a molecular mass of 15.2 kDa. The apparent molecular mass of the recombinant protein is approximately 27-35 kDa in SDS-PAGE under reducing conditions due to glycosylation.

> 90 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA.
Immobilized Rat CD47-His at 10 μg/ml (100 μl/well) can bind mouse SIRPA-Fc (Cat:503641), The EC₅₀ of mouse SIRPA-Fc (Cat:503641) is 0.6-1.4 μg/ml.