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Product Name
Anti-CD47 antibody
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Description
Rabbit polyclonal to CD47
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Tested applications
ELISA, WB
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Species reactivity
Rat CD47
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Alternative names
IAP antibody; 9130415E20Rik antibody; AA407862 antibody; AI848868 antibody; antigen identified by monoclonal antibody 1D8 antibody; antigenic surface determinant protein OA3 antibody; AW108519 antibody; B430305P08Rik antibody; CD47 antigen (Rh-related antigen antibody; CD47 glycoprotein antibody; IAP antibody; IAP antibody; integrin associated protein antibody; integrin-associated protein antibody; integrin-associated signal transducer antibody; integrin-associated signal transducer) antibody; Itgp antibody; leukocyte surface antigen CD47 antibody; leukocyte surface antigen CD47 antibody; MER6 antibody; OA3 antibody; Rh-related antigen antibody; Rh-related antigen antibody; IAP antibody; Itgp antibody; AA407862 antibody; AI848868 antibody; AW108519 antibody; 9130415E20Rik antibody; B430305P08Rik antibody; OA3 antibody; MER6 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Rat CD47 (rh CD47; P97829-1; Met1-Lys140). CD47 specific IgG was purified by Rat CD47 affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 5-10 μg/ml
ELISA:0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Rat CD47. The detection limit for Rat CD47 is < 0.039 ng/well.
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Validations
CD47 Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
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Background
CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain and is a receptor for the C-terminal cell binding domain of thrombospondin. It may play a role in membrane transport and signal transduction. CD47 is also a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. It is very broadly distributed on normal adult tissues, as well as ovarian tumors, being especially abundant in some epithelia and the brain. CD47 may play a role in membrane transport and/or integrin dependent signal transduction. It may prevent premature elimination of red blood cells. It also may be involved in membrane permeability changes induced following virus infection. By acting as an adhesion receptor for THBS1 on platelets, CD47 plays a role in both cell adhesion and in the modulation of integrins. It also plays an important role in memory formation and synaptic plasticity in the hippocampus.
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References
- Brown EJ, et al. (2001) Integrin-associated protein (CD47) and its ligands. Trends Cell Biol. 11(3): 130-5.
- Oldenborg PA. (2004) Role of CD47 in erythroid cells and in autoimmunity. Leuk Lymphoma. 45(7): 1319-27.
- Kaczorowski DJ, et al. (2007) Targeting CD47: NO limit on therapeutic potential. Circ Res. 100(5): 602-3.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"