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Product Name
Human Leptin recombinant protein
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Description
May function as part of a signaling pathway that acts to regulate the size of the body fat depot.
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Protein name
Leptin
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Protein short names
OB; LEPTIN; LEP; OBS; OBESE; LEPD; FLJ94114
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Uniprot ID
P41159
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Gene Name
LEP; hCG_33000; tcag7.84
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Source/Expression Host
E. coli
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Expression Plasmid/cDNA
A DNA sequence encoding the mature form of human Leptin (NP_000221.1) (Val 22-Cys 167) was expressed, with an additional Met.
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Protein Species
Human
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Molecular weight
The recombinant human leptin consists of 147 amino acids and predicts a molecular mass of 16 kDa. The apparent molecular mass of rhLEP is approximately 13 kDa in SDS-PAGE under reducing conditions.
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Purity
> 98 % as determined by SDS-PAGE
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Activity
Measured by its binding ability in a functional ELISA.
1. Immobilized human Leptin at 1.25 μg/ml (100 μl/well) can bind human Leptin receptor Fc chimera with a linear range of 0.032-4.0 μg/ml.
2. Immobilized human Leptin at 5 μg/ml (100 μl/well) can bind human Leptin receptor his with a linear range of 0.032-4.0 μg/ml. -
Validations
Human Leptin Protein SDS-PAGE
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