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Product Name
ISCU antibody
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Description
ISCU Rabbit Polyclonal antibody. Positive IP detected in mouse heart tissue. Positive WB detected in mouse heart tissue, COLO 320 cells, HeLa cells. Positive IHC detected in human lung cancer tissue, human endometrial cancer tissue. Positive IF detected in MCF-7 cells. Observed molecular weight by Western-blot: 14-15 kDa,18-19 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
2310020H20Rik antibody; HML antibody; hnifU antibody; ISCU antibody; ISU2 antibody; NIFU antibody; NifU like protein antibody; NIFUN antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ISCU recombinant protein (Accession Number: NM_213595). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
mouse heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:111936(ISCU antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:111936(ISCU antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of MCF-7 cells, using ISCU antibody Catalog No:111936 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-ISCU (IP:Catalog No:111936, 3ug; Detection:Catalog No:111936 1:500) with mouse heart tissue lysate 4000ug.
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Background
Iron-sulfur (Fe-S) clusters are required for the functions of mitochondrial aconitase, mammalian iron regulatory protein 1, and many other proteins in multiple subcellular compartments. Mitochondrial and cytosolic isoforms of the human Fe-S cluster scaffold protein, iron-sulfur cluster assembly enzyme (ISCU), is involved in activation of iron uptake, redistribution of intracellular iron, and decreased utilization of iron in Fe-S proteins. Defects in ISCU gene are the cause of myopathy with exercise intolerance Swedish type (MEIS), which is characterized by lifelong severe exercise intolerance (PMID: 18304497). Two ISCU isoforms ISCU1 (cytosolic, 15 kDa) and ISCU2 (mitochondrial, 18 kDa), were produced by alternative splicing, and ISCU2 is considered the canonical isoform1. ISCU2 protein is rapidly processed upon maturation (PMID: 11060020). Proteintech’s ISCU antibody 14812-1-AP is raised against the full-length ISCU2 and is expected to detect 14 kDa,15 kDa and 18-19 kDa bands which has been reported in the previous observation (PMID: 11060020).
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References
- Ferecatu I, Gonçalves S, Golinelli-Cohen MP. The diabetes drug target MitoNEET governs a novel trafficking pathway to rebuild an Fe-S cluster into cytosolic aconitase/iron regulatory protein 1. The Journal of biological chemistry. 289(41):28070-86. 2014.
- Stehling O, Vashisht AA, Mascarenhas J. MMS19 assembles iron-sulfur proteins required for DNA metabolism and genomic integrity. Science (New York, N.Y.). 337(6091):195-9. 2012.
- Funauchi Y, Tanikawa C, Yi Lo PH. Regulation of iron homeostasis by the p53-ISCU pathway. Scientific reports. 5:16497. 2015.
- Maio N, Singh A, Uhrigshardt H, Saxena N, Tong WH, Rouault TA. Cochaperone binding to LYR motifs confers specificity of iron sulfur cluster delivery. Cell metabolism. 19(3):445-57. 2014.
- Qiao A, Khechaduri A, Kannan Mutharasan R, Wu R, Nagpal V, Ardehali H. MicroRNA-210 decreases heme levels by targeting ferrochelatase in cardiomyocytes. Journal of the American Heart Association. 2(2):e000121. 2013.
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