Human ISCU Recombinant protein (GST tag)

Human ISCU Recombinant protein (GST tag)

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Scaffold protein for the de novo synthesis of iron-sulfur (Fe-S) clusters within mitochondria, which is required for maturation of both mitochondrial and cytoplasmic [2Fe-2S] and [4Fe-4S] proteins (PubMed:11060020). First, a [2Fe-2S] cluster is transiently assembled on the scaffold protein ISCU. In a second step, the cluster is released from ISCU, transferred to a glutaredoxin GLRX5, followed by the formation of mitochondrial [2Fe-2S] proteins, the synthesis of [4Fe-4S] clusters and their target-specific insertion into the recipient apoproteins. Cluster assembly on ISCU depends on the function of the cysteine desulfurase complex NFS1-LYRM4/ISD11, which serves as the sulfur donor for cluster synthesis, the iron-binding protein frataxin as the putative iron donor, and the electron transfer chain comprised of ferredoxin reductase and ferredoxin, which receive their electrons from NADH (By similarity). {UniProtKB:Q03020, PubMed:11060020}.

Iron-sulfur cluster assembly enzyme ISCU, mitochondrial

NIFUN

Q9H1K1

ISCU; NIFUN

E. coli

A DNA sequence encoding the human ISCU (NP_998760.1) 1-167 aa was fused with the N-terminal GST tag

Human

Not tested.