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IRF5 antibody

Cat.#: 111837

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Special Price 239.0 USD

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Product Information

  • Product Name
    IRF5 antibody
  • Documents
  • Description
    IRF5 Rabbit Polyclonal antibody. Positive FC detected in HepG2 cells. Positive IHC detected in human breast cancer tissue. Positive IF detected in HepG2 cells. Positive WB detected in Jurkat cells, A549 cells, HeLa cells, human heart tissue, NIH/3T3 cells, SH-SY5Y cells. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 47-50 kDa
  • Tested applications
    ELISA, WB, IHC, IF, FC, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    interferon regulatory factor 5 antibody; IRF 5 antibody; IRF5 antibody; SLEB10 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of IRF5 recombinant protein (Accession Number: XM_047420339). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IP: 1:200-1:1000

    IHC: 1:20-1:200

    IF: 1:10-1:100

  • Validations

    Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:111837(IRF5 antibody) at dilution of 1:800

    Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:111837(IRF5 antibody) at dilution of 1:800

    Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:111837(IRF5 antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:111837(IRF5 antibody) at dilution of 1:50 (under 40x lens)

    IP Result of anti-IRF5 (IP:Catalog No:111837, 4ug; Detection:Catalog No:111837 1:300) with HeLa cells lysate 1600ug.

    IP Result of anti-IRF5 (IP:Catalog No:111837, 4ug; Detection:Catalog No:111837 1:300) with HeLa cells lysate 1600ug.

    Immunofluorescent analysis of HepG2 cells using Catalog No:111837(IRF5 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of HepG2 cells using Catalog No:111837(IRF5 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    1X10^6 HepG2 cells were stained with 0.2ug IRF5 antibody (Catalog No:111837, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

    1X10^6 HepG2 cells were stained with 0.2ug IRF5 antibody (Catalog No:111837, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

  • Background
    IRF5, also named as SLEB10, contians one IRF tryptophan pentad repeat DNA-binding domain and belongs to the IRF family. It is a transcription factor involved in the induction of interferons IFNA and INFB and inflammatory cytokines upon virus infection. It is activated by TLR7 or TLR8 signaling. Genetic variations in IRF5 are associated with susceptibility to inflammatory bowel disease type 14 (IBD14) and systemic lupus erythematosus type 10 (SLEB10). IRF5 is also used as M1 marcrophage lineage marker. Alternative splice variant encoding different isoforms exist. Catalog#10547-1-AP is a rabbit polyclonal antibody raised against the C-terminal of human IRF5.
  • References
    • Xu D, Meyer F, Ehlers E, Blasnitz L, Zhang L. Interferon regulatory factor 4 (IRF-4) targets IRF-5 to regulate Epstein-Barr virus transformation. The Journal of biological chemistry. 286(20):18261-7. 2011.
    • Chang Foreman HC, Van Scoy S, Cheng TF, Reich NC. Activation of interferon regulatory factor 5 by site specific phosphorylation. PloS one. 7(3):e33098. 2012.
    • Mattos A, de Jager-Krikken A, de Haan M, Beljaars L, Poelstra K. PEGylation of interleukin-10 improves the pharmacokinetic profile and enhances the antifibrotic effectivity in CCl₄-induced fibrogenesis in mice. Journal of controlled release : official journal of the Controlled Release Society. 162(1):84-91. 2012.
    • Prins JR, Hylkema MN, Erwich JJ. Smoking during pregnancy influences the maternal immune response in mice and humans. American journal of obstetrics and gynecology. 207(1):76.e1-14. 2012.
    • Xu H, Krolikowski JG, Jones DW. 4F decreases IRF5 expression and activation in hearts of tight skin mice. PloS one. 7(12):e52046. 2012.
    • Binder F, Hayakawa M, Choo MK, Sano Y, Park JM. Interleukin-4-induced β-catenin regulates the conversion of macrophages to multinucleated giant cells. Molecular immunology. 54(2):157-63. 2013.
    • Draijer C, Robbe P, Boorsma CE, Hylkema MN, Melgert BN. Characterization of macrophage phenotypes in three murine models of house-dust-mite-induced asthma. Mediators of inflammation. 2013:632049. 2013.
    • Wang H, Dong D, Tang S, Chen X, Gao Q. PPE38 of Mycobacterium marinum triggers the cross-talk of multiple pathways involved in the host response, as revealed by subcellular quantitative proteomics. Journal of proteome research. 12(5):2055-66. 2013.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"