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Product Name
IRF5 antibody
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Description
IRF5 Mouse Monoclonal antibody. Positive IHC detected in human breast cancer tissue, human tonsillitis tissue. Positive IF detected in HepG2 cells. Positive WB detected in HeLa cells, HepG2 cells. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 63+110 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
interferon regulatory factor 5 antibody; IRF 5 antibody; IRF5 antibody; SLEB10 antibody
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Isotype
Mouse IgG2a
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Preparation
This antibody was obtained by immunization of IRF5 recombinant protein (Accession Number: XM_047420339). Purification method: Protein A purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:107354(IRF5 antibody) at dilution of 1:1000
IP Result of anti-IRF5 (IP:Catalog No:107354, 4ug; Detection:Catalog No:107354 1:500) with HeLa cells lysate 1600ug.
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using Catalog No:107354(IRF5 Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:107354(IRF5 Antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG
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Background
IRF5, also named as SLEB10, contians one IRF tryptophan pentad repeat DNA-binding domain and belongs to the IRF family. I t is a transcription factor involved in the induction of interferons IFNA and INFB and inflammatory cytokines upon virus infection. It is activated by TLR7 or TLR8 signaling. Genetic variations in IRF5 are associated with susceptibility to inflammatory bowel disease type 14 (IBD14) and systemic lupus erythematosus type 10 (SLEB10).
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References
- Martin HJ, Lee JM, Walls D, Hayward SD. Manipulation of the toll-like receptor 7 signaling pathway by Epstein-Barr virus. Journal of virology. 81(18):9748-58. 2007.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"