Marmoset IL17 / IL17a recombinant protein

Marmoset IL17 / IL17a recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

IL17, also known as IL17a, is a cytokine belongs to the IL-17 family. Cytokines are proteinaceous signaling compounds that are major mediators of the immune response. They control many different cellular functions including proliferation, differentiation and cell survival/apoptosis but are also involved in several pathophysiological processes including viral infections and autoimmune diseases. Cytokines are synthesized under various stimuli by a variety of cells of both the innate (monocytes, macrophages, dendritic cells) and adaptive (T- and B-cells) immune systems. The IL-17 family of cytokines includes six members, IL-17/IL-17A, IL-17B, IL-17C, IL-17D, IL-17E/IL-25, and IL-17F, which are produced by multiple cell types. IL-17 regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of IL-17 are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.

Interleukin 17 A

CYTOTOXIC T-LYMPHOCYTE-ASSOCIATED ANTIGEN 8; CYTOTOXIC T-LYMPHOCYTE-ASSOCIATED PROTEIN 8; CTLA8; INTERLEUKIN 17 (CYTOTOXIC T-LYMPHOCYTE-ASSOCIATED SERINE ESTERASE 8); IL17; IL-17A; CTLA-8; IL-17; CYTOTOXIC T-LYMPHOCYTE-ASSOCIATED SERINE ESTERASE 8; INTERLEUKIN-17A; CYTOTOXIC T-LYMPHOCYTE-ASSOCIATED ANTIGEN 8. INTERLEUKIN-17A

A5HJM0

E. coli

A DNA sequence encoding the Marmoset IL17A (ABQ09496.1) (Ile20-Ala155) was expressed and purified with an initial Met.

Marmoset

The recombinant Marmoset IL17A consists of 135 amino acids and has a calculated molecular mass of 15.4 kDa. It migrates as an 17 kDa band in SDS-PAGE under reducing conditions.

> 85 % as determined by SDS-PAGE

1. Measured by its binding ability in a functional ELISA. Immobilized Marmoset IL17A (cat: 502804) at 10 μg/mL (100 μl/well) can bind human IL17Ra (Cat:503306), The EC₅₀ of human IL17Ra (Cat:503306) is 8.5-20.0 ng/mL.
2. Measured by its ability to induce IL-6 secretion by HFF human foreskin fibroblast cells.
The ED₅₀ for this effect is typically 2.95-11.8 ng/mL.