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Product Name
Mouse IL13RA2 / CD213A2 (His & Fc Tag) recombinant protein
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Description
Binds as a monomer with high affinity to interleukin-13 (IL13).
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Protein name
Interleukin-13 receptor subunit alpha-2
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Protein short names
CD213A2; CT19; IL13BP; IL13RA2; IL-13R
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Uniprot ID
O88786
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Gene Name
Il13ra2
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Source/Expression Host
Human Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the extracellular domain (Met 1-Lys 334) of mouse IL13R?2 (NP_032382.1) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
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Protein Species
Mouse
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Molecular weight
The recombinant mouse IL13R?2/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 561 amino acids and has a predicted molecular mass of 64.5 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmIL13R?2/Fc monomer is approximately 75-80 kDa due to glycosylation.
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Purity
> 85 % as determined by SDS-PAGE
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Activity
1. Immobilized human IL13 at 20 μg/ml (100 μl/well) can bind mouse IL13RA2 with a linear ranger of 0.5-2 μg/ml.
2. Measured by its ability to inhibit IL13 dependent proliferation of TF1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140: 323. The ED50 for this effect is typically 0.05-0.15 μg/mL. -
Validations
Mouse IL13RA2 / CD213A2 Protein (His & Fc Tag) SDS-PAGE
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