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Product Name
Anti-IL13RA2 / CD213A2 antibody
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Description
Rabbit polyclonal to IL13RA2 / CD213A2
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Tested applications
ELISA, IHC-P
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Species reactivity
Rat IL13RA2 / IL13R
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Alternative names
CD213A2 antibody; CD213a2 antibody; CT19 antibody; IL13BP antibody; IL-13R antibody; IL13RA2 antibody; Il13ra2 antibody; CT19 antibody; IL-13R antibody; IL13BP antibody; CD213A2 antibody; CD213a2 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Rat IL13RA2 / IL13R (rR IL13RA2 / IL13R; Q8VHK6; Met1-Lys336). IL13RA2 / IL13R specific IgG was purified by Rat IL13RA2 / IL13R affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Rat IL13RA2 / IL13R. The detection limit for Rat IL13RA2 / IL13R is approximately 0.00245 ng/well.
IHC-P: 0.1-2 μg/mL
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Validations
IL13RA2 / IL13R Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of rat IL13RA2 in rat brain with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).
IL13RA2 / IL13R Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of rat IL13RA2 in rat liver with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).
IL13RA2 / IL13R Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of rat IL13RA2 in rat spleen with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).
IL13RA2 / IL13R Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of rat IL13RA2 in rat stomach with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).
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Background
Interleukin-13 receptor subunit alpha-2 (IL13RA2/IL-13RA2) is also known as also known as cluster of differentiation 213A2 (CD213A2), IL-13 receptor subunit alpha-2, IL-13R subunit alpha-2, and IL-13RA2. The IL13RA2 is often overexpressed in brain tumors, making Il13ra2 one of the vaccine targets for immunotherapy of glioma. IL13RA2/IL-13RA2 is a cancer-associated receptor that is present in greater than 80% of High Grade Astrocytomas (HGA) and has recently been recognized as a cytokine that predisposes breast cancer cells to metastasize. Expression of IL13Rα2 was rapidly lost from the surface of transduced cells grown in culture. The loss appeared to be related to ligands present in fetal bovine serum in the medium. None of the malignant glioma cell lines cultivated in vitro and tested to date exhibited the IL13Rα2 receptor. A recombinant virus (R5111) enters cells via its interaction with the IL13Rα2 receptor in a manner that cannot be differentiated from the interaction of wild-type virus with its receptors.
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References
- Zhou G, et al.. (2005) Characterization of a recombinant herpes simplex virus 1 designed to enter cells via the IL13Ralpha2 receptor of malignant glioma cells. J Virol. 79(9): 5272-7.
- Osawa M, et al.. (2000) Characterization of the mouse interleukin-13 receptor alpha1 gene. Immunogenetics. 51(11): 974-81.
- Nair BG, et al.. (2011) Nanotechnology platforms; an innovative approach to brain tumor therapy. Med Chem. 7(5): 488-503.
- Benson M, et al.. (2006) A network-based analysis of the late-phase reaction of the skin. J Allergy Clin Immunol. 118(1): 220-5.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"