Mouse IL-28A / IL28A (His Tag) recombinant protein

Mouse IL-28A / IL28A (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Cytokine with antiviral, antitumour and immunomodulatory activities. Plays a critical role in the antiviral host defense, predominantly in the epithelial tissues. Acts as a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1, and receptor engagement leads to the activation of the JAK/STAT signaling pathway resulting in the expression of IFN-stimulated genes (ISG), which mediate the antiviral state. Has a restricted receptor distribution and therefore restricted targets: is primarily active in epithelial cells and this cell type-selective action is because of the epithelial cell-specific expression of its receptor IFNLR1. Seems not to be essential for early virus-activated host defense in vaginal infection, but plays an important role in Toll-like receptor (TLR)-induced antiviral defense. Plays a significant role in the antiviral immune defense in the intestinal epithelium. Exerts an immunomodulatory effect by up-regulating MHC class I antigen expression.

Interferon lambda-2

ZCYTO20; EG330496; IL28A; IL-28A

Q4VK74

Ifnl2; Il28a

Human Cells

A DNA sequence encoding the mouse IL28A (NP_001019844.2) (Met1-Val193) was expressed with a C-terminal polyhistidine tag.

Mouse

The recombinant mouse IL28A comprises 185 amino acids and has a predicted molecular mass of 21.1 kDa. The apparent molecular mass of the protein is approximately 28.5 and 24.7 kDa in SDS-PAGE under reducing conditions.

(82.5+15.3) % as determined by SDS-PAGE

Measured in antiviral assays using HepG2 cells infected with vesicular stomatitis virus.
The ED₅₀ for this effect is typically 1-4 ng/mL.