Rat IFN gamma (Fc Tag) recombinant protein

Rat IFN gamma (Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.

Interferon gamma

IFNG; IFN GAMMA; IFI; GAMMA INTERFERON; IFN-G; IMMUNE INTERFERON; IFG; INTERFERON GAMMA

P01581

Ifng

Human Cells

A DNA sequence encoding the rat IFNG (P01581) (Met 1-Cys 156) was fused with the Fc region of human IgG1 at the C-terminus.

Rat

The secreted recombinant rat IFNG/Fc is a disulfide-linked homodimer. The reduced monomer comprises 375 amino acids and predicts a molecular mass of 42.5 kDa. The apparent molecular mass of the rat IFNG/Fc monomer is approximately 52 kDa in SDS-PAGE under reducing conditions.

> 92 % as determined by SDS-PAGE

1. Measured in antiviral assays using L929 cells infected with vesicular stomatitisvirus (VSV). The ED₅₀ for this effect is typically 1-5 ng/mL.
2. Measured by its binding ability in a functional ELISA. Immobilized rat IFNG-Fc (cat:500370) at 10 μg/ml (100 μl/well) can bind biotinylated rat IFNGR-Fc (Cat:502417). The EC₅₀ of biotinylated rat IFNGR-Fc (Cat:502417) is 20.1-46.9 ng/ml.