Mouse IFN gamma (Fc Tag) recombinant protein

Mouse IFN gamma (Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

IFN gamma, also known as IFNG, is a secreted protein which belongs to the type I I interferon family. IFN gamma is produced predominantly by natural killer and natural killer T cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte effector T cells once antigen-specific immunity develops. IFN gamma has antiviral, immunoregulatory, and anti-tumor properties. IFNG, in addition to having antiviral activity, has important immunoregulatory functions, it is a potent activator of macrophages, and has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. The IFNG monomer consists of a core of six α-helices and an extended unfolded sequence in the C-terminal region. IFN gamma is critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. Aberrant IFN gamma expression is associated with a number of autoinflammatory and autoimmune diseases. The importance of IFN gamma in the immune system stems in part from its ability to inhibit viral replication directly, and most importantly from its immunostimulatory and immunomodulatory effects. IFNG also promotes NK cell activity.

Interferon gamma

INTERFERON GAMMA; IFG; IMMUNE INTERFERON; IFN-G; GAMMA INTERFERON; IFI; IFN GAMMA; IFNG

P01580

Ifng

Human Cells

A DNA sequence encoding the mouse IFNG (P01580) (Met 1-Cys 155) was fused with the Fc region of human IgG1 at the C-terminus.

Mouse

The secreted recombinant mouse IFNG/Fc is a disulfide-linked homodimer. The reduced monomer comprises 374 amino acids and has a calculated molecular mass of 42.5 kDa. As a result of glycosylation, the apparent molecular mass of the monomer is approximately 55 kDa in SDS-PAGE under reducing conditions.

> 92 % as determined by SDS-PAGE

1. Measured in antiviral assays using L929 cells infected with vesicular stomatitisvirus (VSV). The ED₅₀ for this effect is typically 20-80 ng/mL.
2. Measured by its ability to bind with recombinant mouse IFNGR1-His (Cat:502661) in a functional ELISA.