Human ICOS/AILIM/CD278 (His & Fc Tag) recombinant protein

Human ICOS/AILIM/CD278 (His & Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Inducible costimulator (ICOS), also called AILIM (activiation-inducible lymphocyte immunomediatory molecule) is a cell-surface receptor, and belongs to the CD28 family of immune costimulatory receptors consisting of CD28, CTLA-4 and PD-1. The interaction of B7-H2/ICOS plays a critical role in Th cell differentiation, T−B cell interactions which is essential for germinal center formation, and humoral immune responses, and as well as the production of cytokine IL-4. In addition, ICOS is more potent in the induction of IL-10 production, a cytokine important for suppressive function of T regulatory cells. The B7-1/B7-2--CD28/CTLA-4 and ICOS-B7RP-1 pathway provides key second signals that can regulate the activation, inhibition and fine-tuning of T-lymphocyte responses. ICOS stimulates both Th1 and Th2 cytokine production but may have a preferential role in Th2 cell development. Moreover, The B7-1/B7-2-CD28/CTLA-4 and ICOS-B7RP-1 pathway has been suggested of being involved in the development of airway inflammation and airway hyperresponsiveness.

Inducible T-cell co-stimulator

LY115; ICOS; AILIM; H4; CVID1; MGC39850; CD278; CH29-120A16.4; CRP-1; CCLP

Q9Y6W8

ICOS; hCG_1642889

Human Cells

A DNA sequence encoding the extracellular domain of human ICOS (NP_036224.1) (Met 1-Phe 141) was fused to the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

Human

The recombinant human ICOS/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 368 amino acids and predicts a molecular mass of 41.6 kDa. The apparent molecular mass of rhICOS/Fc monomer is approximately 50 kDa due to glycosylation.

> 94 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA. Immobilized human human B7-H2 at 1 μg/ml (100 μl/well) can bind human ICOS with a linear range of 1.6-200 ng/ml.