Human OX40/CD134 (His & Fc Tag) recombinant protein

Human OX40/CD134 (His & Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

OX40 (CD134) and its binding partner, OX40L (CD252), are members of the tumor necrosis factor receptor/tumor necrosis factor superfamily, is known to break an existing state of tolerance in malignancies, leading to a reactivation of antitumor immunity. The interaction between OX40 and OX40L plays an important role in antigen-specific T-cell expansion and survival. OX40 and OX40L also regulate cytokine production from T cells, antigen-presenting cells, natural killer cells, and natural killer T cells, and modulate cytokine receptor signaling. In line with these important modulatory functions, OX40-OX40L interactions have been found to play a central role in the development of multiple inflammatory and autoimmune diseases, making them attractive candidates for intervention in the clinic. Conversely, stimulating OX40 has shown it to be a candidate for therapeutic immunization strategies for cancer and infectious disease.

Tumor necrosis factor receptor superfamily member 4

OX40; LY-70; TXGP1; IMD16; RP5-902P8.3; ACT35; TNFRSF4; CD134; TXGP1L

P43489

TNFRSF4; TXGP1L

Human Cells

A DNA sequence encoding the extracellular domain (Met 1-Ala 216) of human TNFRSF4 (NP_003318.1) precursor was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

Human

The recombinant human TNFRSF4/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 436 amino acids and has a predicted molecular mass of 48.2 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh TNFRSF4/Fc monomer is approximately 68 kDa due to glycosylation.

> 85 % as determined by SDS-PAGE

Immobilized Cynomolgus mFc-TNFSF4 (Cat:501369) at 10 μg/ml (100 μl/well) can bind human TNFRSF4-Fch, The EC₅₀ of human TNFRSF4-Fch is 0.23-0.55 μg/ml.