Human IL-27 (His Tag) recombinant protein

Cat.#: 504705

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Product Information

  • Product Name
    Human IL-27 (His Tag) recombinant protein
  • Documents
  • Description
    IL-27 protein is a member of the IL-6 superfamily, which is expressed on monocytes, endothelial cells and dendritic cells. IL-27 protein is also referred as the IL-12 p35-related protein, p28, is one subunit of a heterodimeric cytokine complex, and associates with another subunit EBI3 (EBV-induced gene 3), an IL-12 p40-related protein (IL-27B). IL-27 protein is an early product of activated antigen-presenting cells and drives rapid clonal expansion of naive CD4(+) T cells and plays a role in the early regulation of Th1 cells initiation which drives efficient adaptive immune response. IL-27 protein has an antiproliferative activity on melanomas through WSX-1/STAT1 signaling. Thus, IL-27 protein may be an attractive candidate as an antitumor agent applicable to cancer immunotherapy.
  • Protein short names
    IL-27; IL27P28; P28; IL-27A; IL27; MGC71873; EBI-3; IL30; IL-27B; IL-27P28; IL27A; EBI3
  • Uniprot ID
    Q14213
  • Source/Expression Host
    CHO Cells
  • Expression Plasmid/cDNA
    A DNA sequence encoding the IL27 complex composed of IL27B subunit (NP_005746.2) (Met 1-Lys 229) and the N-terminally truncated IL27p28 (IL27A) subunit (NP_663634.2) (Phe 29-Pro 243) linked by an polypeptide linker was fused with a polyhistidine tag at the C-terminus.
  • Protein Species
    Human
  • Molecular weight
    The recombinant human IL27 complex (IL27B & IL27A) consists of 450 amino acids after removal of the signal peptide and has a calculated molecular mass of 56 KDa as estimated in SDS-PAGE under reducing conditions.
  • Purity
    > 90 % as determined by SDS-PAGE
  • Activity
    1. Measured by its binding ability in a functional ELISA. Immobilized human IL27-His at 10 μg/ml (100 μl/well) can bind human IL27RA-Fc (Cat:501241), The EC50 of human IL27RA-Fc (Cat:501241) is 0.2-0.46 μg/ml.
    2. Measured in antiviral assays using HepG2 cells infected with vesicular stomatitisvirus(VSV). The ED50 for this effect is typically 23-92 ng/mL.
  • Validations

    Human IL27 / Interleukin-27 Protein (His Tag) SDS-PAGE

    Human IL27 / Interleukin-27 Protein (His Tag) SDS-PAGE

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"