Human IL-27 (His Tag) recombinant protein

Human IL-27 (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

IL-27 protein is a member of the IL-6 superfamily, which is expressed on monocytes, endothelial cells and dendritic cells. IL-27 protein is also referred as the IL-12 p35-related protein, p28, is one subunit of a heterodimeric cytokine complex, and associates with another subunit EBI3 (EBV-induced gene 3), an IL-12 p40-related protein (IL-27B). IL-27 protein is an early product of activated antigen-presenting cells and drives rapid clonal expansion of naive CD4(+) T cells and plays a role in the early regulation of Th1 cells initiation which drives efficient adaptive immune response. IL-27 protein has an antiproliferative activity on melanomas through WSX-1/STAT1 signaling. Thus, IL-27 protein may be an attractive candidate as an antitumor agent applicable to cancer immunotherapy.

IL-27; IL27P28; P28; IL-27A; IL27; MGC71873; EBI-3; IL30; IL-27B; IL-27P28; IL27A; EBI3

Q14213

CHO Cells

A DNA sequence encoding the IL27 complex composed of IL27B subunit (NP_005746.2) (Met 1-Lys 229) and the N-terminally truncated IL27p28 (IL27A) subunit (NP_663634.2) (Phe 29-Pro 243) linked by an polypeptide linker was fused with a polyhistidine tag at the C-terminus.

Human

The recombinant human IL27 complex (IL27B & IL27A) consists of 450 amino acids after removal of the signal peptide and has a calculated molecular mass of 56 KDa as estimated in SDS-PAGE under reducing conditions.

> 90 % as determined by SDS-PAGE

1. Measured by its binding ability in a functional ELISA. Immobilized human IL27-His at 10 μg/ml (100 μl/well) can bind human IL27RA-Fc (Cat:501241), The EC₅₀ of human IL27RA-Fc (Cat:501241) is 0.2-0.46 μg/ml.
2. Measured in antiviral assays using HepG2 cells infected with vesicular stomatitisvirus(VSV). The ED₅₀ for this effect is typically 23-92 ng/mL.