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Product Name
Anti-IL-27 antibody
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Description
Rabbit polyclonal to IL-27
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Tested applications
ELISA, WB, IP
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Species reactivity
Human IL-27
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Alternative names
IL30 antibody; IL27A antibody; IL-27A antibody; IL27p28 antibody; p28 antibody; Il30 antibody; IL-27p28 antibody; IL-27 antibody; EBI3 antibody; IL-27B antibody; Ebi3 antibody; EBI-3 antibody; IL-27 antibody; P28 antibody; Il27 antibody; IL27 antibody; IL-27 antibody; IL-27A antibody; IL27p28 antibody; IL-27p28 antibody; Il30 antibody; IL30 antibody; MGC71873 antibody; p28 antibody; p28 antibody; IL-27 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Human IL-27 (rh IL-27; NP_005746.2 & NP_663634.2; Met1-Lys229 & Phe29-Pro243). IL-27 specific IgG was purified by Human IL-27 affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 10-20 μg/ml
ELISA: 0.1-0.2 μg/ml
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human IL-27.
IP: 4-6 μg/mg of lysate
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Validations
IL-27 Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
IL-27 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunoprecipitation
Immunochemical staining of human E-cadherin in human gastric cancer with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).
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Background
IL-27 protein is a member of the IL-6 superfamily, which is expressed on monocytes, endothelial cells and dendritic cells. IL-27 protein is also referred as the IL-12 p35-related protein, p28, is one subunit of a heterodimeric cytokine complex, and associates with another subunit EBI3 (EBV-induced gene 3), an IL-12 p40-related protein (IL-27B). IL-27 protein is an early product of activated antigen-presenting cells and drives rapid clonal expansion of naive CD4(+) T cells and plays a role in the early regulation of Th1 cells initiation which drives efficient adaptive immune response. IL-27 protein has an antiproliferative activity on melanomas through WSX-1/STAT1 signaling. Thus, IL-27 protein may be an attractive candidate as an antitumor agent applicable to cancer immunotherapy.
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References
- Hisada M, et al. (2004) Potent antitumor activity of interleukin-27. Cancer Res. 64(3): 1152-6.
- Larousserie F, et al. (2005) Analysis of interleukin-27 (EBI3/p28) expression in Epstein-Barr virus- and human T-cell leukemia virus type 1-associated lymphomas: heterogeneous expression of EBI3 subunit by tumoral cells. Am J Pathol. 166(4): 1217-28.
- Seita J, et al. (2008) Interleukin-27 directly induces differentiation in hematopoietic stem cells. Blood. 111(4): 1903-12.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"