Human IL-1RA/IL1RN recombinant protein

Human IL-1RA/IL1RN recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Interleukin-1 receptor antagonist (IL-1RA) also known as IL1RN is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses. A polymorphism of this protein encoding gene is reported to be associated with increased risk of osteoporotic fractures and gastric cancer. IL-1RA/IL1RN may inhibit the activity of IL-1 by binding to its receptor and it has no IL-1 like activity. Genetic variation in IL-1RA/IL1RN is associated with susceptibility to microvascular complications of diabetes type 4 (MVCD4). These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Defects in IL-1RA/IL1RN are the cause of interleukin 1 receptor antagonist deficiency (DIRA) which is also known as deficiency of interleukin 1 receptor antagonist. Autoinflammatory diseases manifest inflammation without evidence of infection, high-titer autoantibodies, or autoreactive T-cells. DIRA is a rare, autosomal recessive, genetic autoinflammatory disease that results in sterile multifocal osteomyelitis, and pustulosis from birth.

Interleukin-1 receptor antagonist protein

IL-1RA; DIRA; IL-1RN; IL-1RA3; RP23-218B17.2; IRAP; ICIL-1RA; IL1RN; MGC10430; IL1F3; IL1RA; MVCD4; F630041P17RIK

P18510

IL1RN; IL1F3; IL1RA

E. coli

A DNA sequence encoding the mature form of human IL1RA isoform1 (NP_776214.1) (Arg 26-Glu 177) was expressed.

Human

The recombinant human IL1RA consists of 153 amino acids and predicts a molecular mass of 17.3 kDa which is also estimated by SDS-PAGE.

> 97 % as determined by SDS-PAGE

1. Measured by its binding ability in a functional ELISA. Immobilized human IL1RA at 10 μg/ml (100 μl/well) can bind human IL1R2-Fc (Cat:504536), The EC₅₀ of human IL1R2-Fc (Cat:504536) is 0.04-0.1 μg/mL.
2. Measured by its binding ability in a functional ELISA. Immobilized human IL1RA at 10 μg/ml (100 μl/well) can bind human IL1R1-Fc (Cat:500060), The EC₅₀ of human IL1R1-Fc (Cat:500060) is 0.08-0.2 μg/mL.