Human HER3/ErbB3 (Fc Tag) recombinant protein

Human HER3/ErbB3 (Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

ErbB3, also known as Her3(human epidermal growth factor receptor3), is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound glycoprotein has a neuregulin binding domain but has not an active kinase domain., and therefore can not mediate the intracellular signal transduction through protein phosphorylation. However, its heterodimer with ErbB2 or other EGFR members responsible for tyrosine phosphorylation forms a receptor complex with high affinity, and initiates the related pathway which lead to cell proliferation or differentiation. ErbB3 has been shown to implicated in numerous cancers, including prostate, bladder, and breast tumors. This protein has different isoforms derived from alternative splicing variants, and among which, the secreted isoform lacking the intermembrane region modulates the activity of membrane-bound form.

Receptor tyrosine-protein kinase erbB-3

ERBB3R; LCCS2; MGC88033; ERBB3-S; C76256; P180-ERBB3; HER3; C-ERBB-3; MDA-BF-1; C-ERBB3; MGC117742; P85-SERBB3; P45-SERBB3; ERBB3; ERBB-3

P21860

ERBB3; HER3

Human Cells

A DNA sequence encoding the human ErbB3 (NP_001973.2) (Met1-Thr643)was fused with Fc region of mouse IgG at the C-terminus.

Human

The recombinant human ErbB3/mFc is a disulfide-linked homodimer. The reduced monomer comprises 858 amino acids and has a predicted molecular mass of 95 kDa. The apparent molecular mass of the protein is approximately 119-129 in SDS-PAGE under reducing conditions.

> 95 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA.
Immobilized human ErbB3-mFc at 10 μg/ml (100 μl/well) can bind biotinylated human NRG1 (Cat:503891), The EC₅₀ of biotinylated human NRG1 (Cat:503891) is 0.58-1.25 μg/ml.