Human Ephrin-A1/EFNA1 (His & Fc Tag) recombinant protein

Human Ephrin-A1/EFNA1 (His & Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

EPH-related receptor tyrosine kinase ligand 1 (abbreviated as Ephrin-A1) also known as ligand of eph-related kinase 1 or EFNA1, is a member of the ephrin (EPH) family. The Eph family receptor interacting proteins (ephrins) are a family of proteins that serve as the ligands of the Eph receptor, which compose the largest known subfamily of receptor protein-tyrosine kinases (RTKs). Ephrin-A1/EFNA1 and its Eph family of receptor tyrosine kinases are expressed by cells of the SVZ. Ephrin subclasses are further distinguished by their mode of attachment to the plasma membrane: ephrin-A ligands bind EphA receptors and are anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) linkage, whereas ephrin-B ligands bind EphB receptors and are anchored via a transmembrane domain. An exception is the EphA4 receptor, which binds both subclasses of ephrins. Ephrin-A1 and one of its receptor EphA2 were expressed in xenograft endothelial cells and also tumor cells and play a role in human cancers, at least in part by influencing tumor neovascularization.

Ephrin-A1

EFNA1; EFL1; B61; EPHRIN A1; TNFAIP4; AI325262; EPLG1; EPHRIN-A1; LERK-1; LERK1; ECKLG; EPL1

P20827

EFNA1; EPLG1; LERK1; TNFAIP4

Human Cells

A DNA sequence encoding the human EphrinA1 (NP_004419.2) without the propeptide (Met1-Ser182) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

Human

The recombinant human EphrinA1/Fc chimera is a disulfide-linked homodimer. The reduced monomer comprises 412 amino acids a predicted molecular mass of 47.5 kDa. As a result of glycosylation, rh EphrinA1/Fc monomer migrates as an approximately 50-55 kDa band in SDS-PAGE under reducing conditions.

> 85 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA. Immobilized mouse EphA2 at 2 μg/ml (100 μl/well) can bind human EphrinA1 with a linear range of 0.8-20 ng/ml.