Rat Ephrin-A1/EFNA1 (His Tag) recombinant protein

Rat Ephrin-A1/EFNA1 (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. Plays an important role in angiogenesis and tumor neovascularization. The recruitment of VAV2, VAV3 and PI3-kinase p85 subunit by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly. Exerts anti-oncogenic effects in tumor cells through activation and down-regulation of EPHA2. Activates EPHA2 by inducing tyrosine phosphorylation which leads to its internalization and degradation. Acts as a negative regulator in the tumorigenesis of gliomas by down-regulating EPHA2 and FAK. Can evoke collapse of embryonic neuronal growth cone and regulates dendritic spine morphogenesis (By similarity).

Ephrin-A1

EPHRIN-A1; EPLG1; AI325262; TNFAIP4; EPHRIN A1; B61; EFL1; EFNA1; EPL1; ECKLG; LERK1; LERK-1

P97553

Efna1; Epgl1; Lerk1

Human Cells

A DNA sequence encoding the rat EFNA1 (P97553) (Met1-His181) was expressed, fused with a polyhistidine tag at the C-terminus.

Rat

The recombinant rat EFNA1 comprises 175 amino acids and predicts a molecular mass of 20.7 kDa. The apparent molecular mass of the rat EFNA1 is approximately 27 kDa in SDS-PAGE under reducing conditions due to glycosylation.

> 95 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA.
Immobilized Rat EFNA1-His (Cat:501342）at 10 μg/mL (100 μl/well) can bind biotinylated mouse EPHA2-His (Cat:502565) with a linear range of 6.25-200 ng/ml.