Human ENPP7 (His Tag) recombinant protein

Human ENPP7 (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Mouse Ectonucleotide pyrophosphatase / phosphodiesterase family member 7, also known as Alkaline sphingomyelin phosphodiesterase, Intestinal alkaline sphingomyelinase, Alk-Smase, ENPP7 and NPP-7, is a single-pass type I membrane protein which belongs to the nucleotide pyrophosphatase / phosphodiesterase family. ENPP7 / NPP-7 is expressed in the intestines and human bile. ENPP7 / NPP-7 is localized at the surface of the microvillar membrane in small intestine enterocytes, as well as in endosome-like structures and in Golgi complex. The main function of ENPP7 / NPP-7 is to convert the dietary sphingomyelin into ceramide, the sphingolipid messengers via hydrolyzation. ENPP7 / NPP-7 is also reported to exert a phospholipase C activity toward palmitoyl lyso-phosphocholine. The activity of this enzyme is inhibited in a dose dependent manner by ATP, imidazole, orthovanadate and zinc ion. Further, It has been shown in studies that decreased levels of ENPP7 / NPP-7 may be associated with human colon cancer.

ENPP7; GM254; MGC50179; ALK-SMASE; RP23-386D6.4; NPP7; E-NPP7; NPP-7

Q6UWV6

Human Cells

A DNA sequence encoding the human ENPP7 (NP_848638.2) (Met 1-Ser 439) was fused with a C-terminal polyhistidine tag.

Human

The secreted recombinant human ENPP7 comprises 429 amino acids with a predicted molecular mass of 49 kDa. As a result of glycosylation, rh ENPP7 migrates as an approximately 55-60 kDa band in SDS-PAGE under reducing conditions.

> 95 % as determined by SDS-PAGE