Human B7-DC/PD-L2/CD273 (His & Fc Tag) recombinant protein

Human B7-DC/PD-L2/CD273 (His & Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Programmed death ligand 2 (PD-L2), also referred to as B7-DC and CD273, is a member of the B7 family of proteins including B7-1, B7-2, B7-H2, B7-H1 (PD-L1), and B7-H3. PD-L2 is a type I membrane protein and structurally consists of an extracellular region containing one V-like and one C-like Ig domain, a transmembrane region, and a short cytoplasmic domain. PD-L2 is expressed on antigen presenting cells, placental endothelium and medullary thymic epithelial cells, and can be induced by LPS in B cells, INF-γ in monocytes, or LPS plus IFN-γ in dendritic cells. The CD28 and B7 protein families are critical regulators of immune responses. PD-L2 and PD-L1 are two ligands for PD-1, member of the CD28/CTLA4 family expressed on activated lymphoid cells, and thus provide signals for regulating T cell activation and immune tolerance. The interaction of B7-DC/PD-1 exhibited a 2-6-fold higher affinity compared with the interaction of B7-H1/PD-1.

Programmed cell death 1 ligand 2

Q9BQ51

PDCD1LG2; B7DC; CD273; PDCD1L2; PDL2

Human Cells

A DNA sequence encoding the extracellular domain (Met 1-Pro 219) of human PD-L2 (NP_079515.2) was fused with the C-terminal His-tagged Fc region of human IgG1 at the C-terminus.

Human

The recombinant human PD-L2/Fc is a disulfide-linked homodimeric protein after proteolytic removal of the signal peptide. The reduced monomer consists of 447 amino acids and predicts a molecular mass of 50.4 kDa. As a result of glycosylation, the apparent molecular mass of rh PD-L2/Fc monomer is approximately 70-80 kDa in SDS-PAGE under reducing conditions.

> 97 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA. Immobilized human PD1 at 2 μg/ml (100 μl/well) can bind human PD-L2 with a linear ranger of 0.0064-0.16 μg/ml.