Mouse B7-DC/PD-L2/CD273 (Fc Tag) recombinant protein

Mouse B7-DC/PD-L2/CD273 (Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Programmed death ligand 2 (PD-L2), also referred to as B7-DC and CD273, is a member of the B7 family of proteins including B7-1, B7-2, B7-H2, B7-H1 (PD-L1), and B7-H3. PD-L2 is a type I membrane protein and structurally consists of an extracellular region containing one V-like and one C-like Ig domain, a transmembrane region, and a short cytoplasmic domain. PD-L2 is expressed on antigen presenting cells, placental endothelium and medullary thymic epithelial cells, and can be induced by LPS in B cells, INF-γ in monocytes, or LPS plus IFN-γ in dendritic cells. The CD28 and B7 protein families are critical regulators of immune responses. PD-L2 and PD-L1 are two ligands for PD-1, member of the CD28/CTLA4 family expressed on activated lymphoid cells, and thus provide signals for regulating T cell activation and immune tolerance. The interaction of B7-DC/PD-1 exhibited a 2-6-fold higher affinity compared with the interaction of B7-H1/PD-1.

Programmed cell death 1 ligand 2

F730015O22RIK; PDCD1L2; CD273; MGC142238; MGC142240; PDL2; MGC124040; PDCD1LG2; PD-L2; BTDC; B7DC; MGC124039; B7-DC; BA574F11.2

Q9WUL5

Pdcd1lg2; B7dc; Btdc; Cd273; Pdl2

Human Cells

A DNA sequence encoding the extracellular domain of mouse PDCD1LG2 (Q9WUL5) (Met 1-Arg 219) was fused with the Fc region of human IgG1 at the C-terminus.

Mouse

The recombinant mouse PDCD1LG2/Fc is a disulfide-linked homodimer. The reduced monomer comprises 441 amino acids and has a calculated molecular mass of 49.5 kDa. As a result of glycosylation, the apparent molecular mass of the monomer is approximately 60 kDa in SDS-PAGE under reducing conditions.

> 95 % as determined by SDS-PAGE

1. Measured by its binding ability in a functional ELISA.
2. Immobilized human PD1-His (Cat:503606) at 10 μg/mL (100 μl/well) can bind mouse PD-L2-Fc, The EC₅₀ of mouse PD-L2-Fc is 0.95 μg/mL.