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Product Name
GDI2 antibody
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Description
GDI2 Mouse Monoclonal antibody. Positive WB detected in Hepg2 cells. Positive IF detected in MCF-7 cells, Hela cells. Positive IHC detected in human breast cancer tissue, human liver cancer tissue. Observed molecular weight by Western-blot: 46 kDa
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Tested applications
ELISA, WB, IF, IHC
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
FLJ16452 antibody; FLJ37352 antibody; GDI 2 antibody; GDI2 antibody; GDP dissOCiation inhibitor 2 antibody; Rab GDI beta antibody; RABGDIB antibody
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Isotype
Mouse IgG1
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Preparation
This antibody was obtained by immunization of GDI2 recombinant protein (Accession Number: NM_001494). Purification method: Caprylic acid/ammonium sulfate precipitation.
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Clonality
Monoclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:107292(GDI2 antibody) at dilution of 1:1000
Immunofluorescent analysis of MCF-7 cells, using GDI2 antibody Catalog No: at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red).
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using Catalog No:107292(GDI2 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using Catalog No:107292(GDI2 Antibody) at dilution of 1:50 (under 40x lens)
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Background
GDP dissociation inhibitors (GDIs) are proteins that regulate the GDP-GTP exchange reaction of members of the rab family, GDIs can bind and release GDP-bound Rab proteins from membranes. Two GDI proteins towards different Rab proteins have been identified. GDI1 interacts with almost all of the Rab proteins, while GDI2 interacts with Rabll but not Rab3A. GDI2 distributes ubiquitously, displaying a membrane bound location in perinuclear regions of cells. GDI-2 was thought to be involved in cellular response to insulin. It electrophoreses as a 46kd protein in SDS-PAGE. (PMID: 7929030; PMID: 19570034). This antibody can bind both GDIs for the close sequences.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"