Product Name

GDI2 antibody

Documents

Description

GDI2 Rabbit Polyclonal antibody. Positive WB detected in mouse brain tissue. Positive IHC detected in human pancreas cancer. Observed molecular weight by Western-blot: 48kd

Tested applications

ELISA, WB, IHC

Species reactivity

human, mouse, rat; other species not tested.

Alternative names

GDI2 antibody; FLJ16452 antibody; GDI 2 antibody; RABGDIB antibody; Rab GDI beta antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of GDI2 recombinant protein (Accession Number: NM_001494). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.1% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:200-1:2000

IHC: 1:10-1:100

Validations

mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:110927(GDI2 antibody) at dilution of 1:500

Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:110927(GDI2 antibody) at dilution of 1:10 (under 10x lens)

Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:110927(GDI2 antibody) at dilution of 1:10 (under 40x lens)

Background

GDP dissociation inhibitors (GDIs) are proteins that regulate the GDP-GTP exchange reaction of members of the rab family, GDIs can bind and release GDP-bound Rab proteins from membranes. Two GDI proteins towards different Rab proteins have been identified. GDI1 interacts with almost all of the Rab proteins, while GDI2 interacts with Rabll but not Rab3A. GDI2 distributes ubiquitously, displaying a membrane bound location in perinuclear regions of cells. GDI-2 was thought to be involved in cellular response to insulin. It electrophoreses as a 46kd protein in SDS-PAGE. (PMID: 7929030; PMID: 19570034). This antibody can bind both GDIs for the close sequences.

References

Onda M, Emi M, Yoshida A. Comprehensive gene expression profiling of anaplastic thyroid cancers with cDNA microarray of 25 344 genes. Endocrine-related cancer. 11(4):843-54. 2004.
Sun ZL, Zhu Y, Wang FQ. Serum proteomic-based analysis of pancreatic carcinoma for the identification of potential cancer biomarkers. Biochimica et biophysica acta. 1774(6):764-71. 2007.
Wishart TM, Paterson JM, Short DM. Differential proteomics analysis of synaptic proteins identifies potential cellular targets and protein mediators of synaptic neuroprotection conferred by the slow Wallerian degeneration (Wlds) gene. Molecular & cellular proteomics : MCP. 6(8):1318-30. 2007.
Machner MP, Isberg RR. A bifunctional bacterial protein links GDI displacement to Rab1 activation. Science (New York, N.Y.). 318(5852):974-7. 2007.
McCray BA, Skordalakes E, Taylor JP. Disease mutations in Rab7 result in unregulated nucleotide exchange and inappropriate activation. Human molecular genetics. 19(6):1033-47. 2010.
Zhu Y, Hu L, Zhou Y, Yao Q, Liu L, Shao F. Structural mechanism of host Rab1 activation by the bifunctional Legionella type IV effector SidM/DrrA. Proceedings of the National Academy of Sciences of the United States of America. 107(10):4699-704. 2010.
Kashyap MK, Harsha HC, Renuse S. SILAC-based quantitative proteomic approach to identify potential biomarkers from the esophageal squamous cell carcinoma secretome. Cancer biology & therapy. 10(8):796-810. 2010.
Orlando R, Borro M, Motolese M. Levels of the Rab GDP dissociation inhibitor (GDI) are altered in the prenatal restrain stress mouse model of schizophrenia and are differentially regulated by the mGlu2/3 receptor agonists, LY379268 and LY354740. Neuropharmacology. 86:133-44. 2014.

GDI2 antibody

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