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FOXO1 antibody

Cat.#: 110703

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Product Information

  • Product Name
    FOXO1 antibody
  • Documents
  • Description
    FOXO1 Rabbit Polyclonal antibody. Positive IHC detected in mouse brain tissue. Positive FC detected in HeLa cells. Positive WB detected in HeLa cells, L02 cells, mouse brain tissue, mouse liver tissue. Observed molecular weight by Western-blot: 70-80 kDa
  • Tested applications
    ELISA, WB, IHC, FC
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    FKH1 antibody; FKHR antibody; forkhead box O1 antibody; Forkhead box protein O1 antibody; Forkhead box protein O1A antibody; Forkhead in rhabdomyosarcoma antibody; FOXO1 antibody; FOXO1A antibody
  • Immunogen
    Human FOXO1 Recombinant protein (GST tag)
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of FOXO1 recombinant protein (Accession Number: NM_002015). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IHC: 1:20-1:200

  • Validations

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:110703(FOXO1 Antibody) at dilution of 1:600

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:110703(FOXO1 Antibody) at dilution of 1:600

    Immunohistochemistry of paraffin-embedded mouse brain tissue slide using Catalog No:110703(FOXO1 Antibody) at dilution of 1:200 (under 40x lens)

    Immunohistochemistry of paraffin-embedded mouse brain tissue slide using Catalog No:110703(FOXO1 Antibody) at dilution of 1:200 (under 40x lens)

    1X10^6 HeLa cells were stained with 0.2ug FOXO1 antibody (Catalog No:110703, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:100.

    1X10^6 HeLa cells were stained with 0.2ug FOXO1 antibody (Catalog No:110703, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:100.

  • Background
    FOXO1, also named as FOXO1A, FKHR and FKH1, is a member of the FOXO subfamily of Forkhead transcription factors. FOXO1 is a transcription factor which acts as a regulator of cell responses to oxidative stress. FOXO1 interacts with LRPPRC and SIRT1. In the presence of KIRT1, FOXO1 mediates down-regulation of cyclin D1 and up-regulation of CDKN1B levels which are required for cell transition from proliferative growth to quiescence. FOXO1 contains three predicted protein kinase B phosphorylation sites (Thr-24, Ser-256, and Ser-319) that are conserved in other FOXO proteins. The t(2;13) and the variant t(1;13) translocations generate PAX3/FKHR and PAX7/FKHR fusion proteins respectively. The resulting protein is a transcriptional activator. Defects in FOXO1 are a cause of rhabdomyosarcoma type 2 (RMS2).
  • References
    • Tolba MF, Esmat A, Al-Abd AM. Caffeic acid phenethyl ester synergistically enhances docetaxel and paclitaxel cytotoxicity in prostate cancer cells. IUBMB life. 65(8):716-29. 2013.
    • Han SM, El Oussini H, Scekic-Zahirovic J. VAPB/ALS8 MSP ligands regulate striated muscle energy metabolism critical for adult survival in caenorhabditis elegans. PLoS genetics. 9(9):e1003738. 2013.
    • Song HM, Luo Y, Li DF. MicroRNA-96 plays an oncogenic role by targeting FOXO1 and regulating AKT/FOXO1/Bim pathway in papillary thyroid carcinoma cells. International journal of clinical and experimental pathology. 8(9):9889-900. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"