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Product Name
FBL antibody
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Description
FBL Rabbit Polyclonal antibody. Positive IF detected in A375 cells. Positive IP detected in A375 cells. Positive WB detected in HeLa cells, A375 cells, mouse kidney tissue, mouse liver tissue. Observed molecular weight by Western-blot: 34 kDa
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Tested applications
ELISA, WB, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
FBL antibody; FIB antibody; FIB1 antibody; fibrillarin antibody; FLRN antibody; RNU3IP1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of FBL recombinant protein (Accession Number: NM_001436). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:110537(FBL antibody) at dilution of 1:500
IP Result of anti-FBL (IP:Catalog No:110537, 4ug; Detection:Catalog No:110537 1:500) with A375 cells lysate 3600ug.
Immunofluorescent analysis of A375 cells using Catalog No:110537(FBL Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
FBL represents fibrillarin
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References
- Szerlong HJ, Herman JA, Krause CM. Proteomic characterization of the nucleolar linker histone H1 interaction network. Journal of molecular biology. 427(11):2056-71. 2015.
- Liu X, Wang X, Du W. Suppressor of fused (Sufu) represses Gli1 transcription and nuclear accumulation, inhibits glioma cell proliferation, invasion and vasculogenic mimicry, improving glioma chemo-sensitivity and prognosis. Oncotarget. 5(22):11681-94. 2014.
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