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FASN antibody

Cat.#: 110529

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Special Price 239.0 USD

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Product Information

  • Product Name
    FASN antibody
  • Documents
  • Description
    FASN Rabbit Polyclonal antibody. Positive WB detected in rat liver tissue, HeLa cells, HepG2 cells, mouse liver tissue. Positive IP detected in mouse liver tissue. Positive IF detected in Hela cells, HepG2 cells. Positive FC detected in HeLa cells. Observed molecular weight by Western-blot: 250-272 kDa
  • Tested applications
    ELISA, IF, IP, WB, FC
  • Species reactivity
    Human, Mouse, Rat; other species not tested.
  • Alternative names
    FAS antibody; FASN antibody; fatty acid synthase antibody; OA 519 antibody; SDR27X1 antibody
  • Immunogen
    Human FASN Recombinant protein (GST tag)
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of FASN recombinant protein (Accession Number: BC007909). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:1000

    IP: 1:200-1:2000

    IF: 1:10-1:100

  • Validations

    Immunofluorescent analysis of Hela cells, using FASN antibody Catalog No:110529 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

    Immunofluorescent analysis of Hela cells, using FASN antibody Catalog No:110529 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

    rat liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:110529(FASN Antibody) at dilution of 1:300

    rat liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:110529(FASN Antibody) at dilution of 1:300

    IP Result of anti-FASN (IP:Catalog No:110529, 4ug; Detection:Catalog No:110529 1:500) with mouse liver tissue lysate 4000ug.

    IP Result of anti-FASN (IP:Catalog No:110529, 4ug; Detection:Catalog No:110529 1:500) with mouse liver tissue lysate 4000ug.

    1X10^6 HeLa cells were stained with 0.2ug FASN antibody (Catalog No:110529, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

    1X10^6 HeLa cells were stained with 0.2ug FASN antibody (Catalog No:110529, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

  • Background
    FASN represents fatty acid synthase
  • References
    • Chen J, Ren J, Jing Q. TSH/TSHR Signaling Suppresses Fatty Acid Synthase (FASN) Expression in Adipocytes. Journal of cellular physiology. 230(9):2233-9. 2015.
    • Cui M, Xiao Z, Wang Y. Long noncoding RNA HULC modulates abnormal lipid metabolism in hepatoma cells through an miR-9-mediated RXRA signaling pathway. Cancer research. 75(5):846-57. 2015.
    • Cai D, Wang J, Jia Y. Gestational dietary betaine supplementation suppresses hepatic expression of lipogenic genes in neonatal piglets through epigenetic and glucocorticoid receptor-dependent mechanisms. Biochimica et biophysica acta. 1861(1):41-50. 2016.
    • Wang MD, Wu H, Fu GB. Acetyl-CoA carboxylase α promotion of glucose-mediated fatty acid synthesis enhances survival of hepatocellular carcinoma in mice and patients. Hepatology (Baltimore, Md.). 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"