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Product Name
Anti-Fatty Acid Synthase (2F9) Mouse antibody
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Description
Fatty Acid Synthase (2F9) Mouse monoclonal antibody
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Tested applications
WB, IP, ICC/IF
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Species reactivity
Human, Mouse, Rat, Monkey
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Alternative names
[Acyl-carrier-protein] S acetyltransferase;[Acyl-carrier-protein] S malonyltransferase;3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase;3-oxoacyl-[acyl-carrier-protein] reductase;3-oxoacyl-[acyl-carrier-protein] synthase;Enoyl-[acyl-carrier-protein] antibody
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Isotype
IgG2a
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Preparation
Antigen: Purified recombinant human Fatty Acid Synthase protein fragments expressed in E.coli.
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Clonality
Monoclonal
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Formulation
Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/1000
;ICC: 1/400
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Validations
Western blot detection of Fatty Acid Synthase in Hela,C6,3T3,CHO-K1 and 293 cell lysates using Fatty Acid Synthase mouse mAb(dilution 1:1000).Predicted band size:273kDa.Observed band size:273kDa.
Immunocytochemistry staining of Hela cells fixed with 4% Paraformaldehyde and using anti-Fatty Acid Synthase mouse mAb (dilution 1:400).
Immunoprecipitation analysis of CHO-K1 cell lysates using Fatty Acid Synthase mouse mAb.
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Background
Swiss-Prot Acc.P49327.Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. This multifunctional protein has 7 catalytic activities and an acyl carrier protein.
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