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Product Name
EPO antibody
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Description
EPO Rabbit Polyclonal antibody. Positive IHC detected in human kidney tissue. Positive WB detected in mouse liver tissue, mouse kidney tissue. Observed molecular weight by Western-blot: 37 kDa, 45 kDa and 80 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
EPO antibody; Epoetin antibody; erythropoietin antibody; MVCD2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of EPO recombinant protein (Accession Number: NM_000799). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IHC: 1:20-1:200
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Validations
mouse liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:110390(EPO Antibody) at dilution of 1:300
Immunohistochemistry of paraffin-embedded human kidney tissue slide using Catalog No:110390(EPO Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human kidney tissue slide using Catalog No:110390(EPO Antibody) at dilution of 1:50 (under 40x lens)
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Background
Erythropoietin (Epo) is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. Its effect is realized by binding erythropoietin receptor (EpoR) expressed on erythroid progenitor cells. EpoR, is a glycoprotein expressed on megakaryocytes, erythroid progenitors and endothelial cells. Epo also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. In western blotting, we got three bands 37 kDa, 45 kDa and 80 kDa. We are unsure as to the identity the extra bands of 45 kDa and 80 kDa.
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