Human EphA2/Eph Receptor A2 (His Tag) recombinant protein

Human EphA2/Eph Receptor A2 (His Tag) recombinant protein

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Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Activated by the ligand ephrin-A1/EFNA1 regulates migration, integrin-mediated adhesion, proliferation and differentiation of cells. Regulates cell adhesion and differentiation through DSG1/desmoglein-1 and inhibition of the ERK1/ERK2 (MAPK3/MAPK1, respectively) signaling pathway. May also participate in UV radiation-induced apoptosis and have a ligand-independent stimulatory effect on chemotactic cell migration. During development, may function in distinctive aspects of pattern formation and subsequently in development of several fetal tissues. Involved for instance in angiogenesis, in early hindbrain development and epithelial proliferation and branching morphogenesis during mammary gland development. Engaged by the ligand ephrin-A5/EFNA5 may regulate lens fiber cells shape and interactions and be important for lens transparency development and maintenance. With ephrin-A2/EFNA2 may play a role in bone remodeling through regulation of osteoclastogenesis and osteoblastogenesis.

Ephrin type-A receptor 2

EPHA2; ECK; AW545284; CTRCT6; RP23-308N2.1; SEK2; ARCC2; SEK-2; CTPP1; MYK2; CTPA

P29317

EPHA2; ECK

Human Cells

A DNA sequence encoding the human EPHA2 (P29317) (Met1-Asn534) was expressed with a polyhistidine tag at the C-terminus.

Human

The recombinant human EPHA2 consists of 522 amino acids and predicts a molecular mass of 57.6 KDa. It migrates as an approximately 65-75 KDa band in SDS-PAGE under reducing conditions.

> 95 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA.
Immobilized human EPHA2-His at 10 μg/ml (100 μl/well) can bind human EFNA1-Fch (Cat:503293), The EC₅₀ of human EFNA1-Fch (Cat:503293) is 0.1-0.2 μg/ml.