Mouse EphA2/Eph Receptor A2 (His Tag) recombinant protein

Mouse EphA2/Eph Receptor A2 (His Tag) recombinant protein

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Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Activated by the ligand ephrin-A1/EFNA1 regulates migration, integrin-mediated adhesion, proliferation and differentiation of cells. Regulates cell adhesion and differentiation through DSG1/desmoglein-1 and inhibition of the ERK1/ERK2 signaling pathway. May also participate in UV radiation-induced apoptosis and have a ligand-independent stimulatory effect on chemotactic cell migration. During development, may function in distinctive aspects of pattern formation and subsequently in development of several fetal tissues. Involved for instance in angiogenesis, in early hindbrain development and epithelial proliferation and branching morphogenesis during mammary gland development. Engaged by the ligand ephrin-A5/EFNA5 may regulate lens fiber cells shape and interactions and be important for lens transparency development and maintenance. With ephrin-A2/EFNA2 may play a role in bone remodeling through regulation of osteoclastogenesis and osteoblastogenesis.

Ephrin type-A receptor 2

EPHA2; ECK; CTRCT6; AW545284; RP23-308N2.1; SEK2; CTPP1; SEK-2; ARCC2; MYK2; CTPA

Q03145

Epha2; Eck; Myk2; Sek2

Human Cells

A DNA sequence encoding the mouse EPHA2 (NP_034269.2) extracellular domain (Met 1-Asn 535) was expressed, fused with a polyhistidine tag at the C-terminus.

Mouse

The secreted recombinant mouse EPHA2 consists of 523 amino acids and has a predicted molecular mass of 58 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmEPHA2 is approximately 65 kDa due to glycosylation.

> 98 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA.
1. Immobilized mouse EphA2 at 2μg/ml (100 μl/well) can bind mouse EphrinA1 with a linear range of 0.16-20 ng/ml.
2. Immobilized mouse EphA2 at 2 μg/ml (100 μl/well) can bind human EphrinA1 with a linear range of 0.8-20 ng/ml.