Rat Ephrin B3 / EFNB3 (His Tag) recombinant protein

Rat Ephrin B3 / EFNB3 (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Ephrin B3 belongs to the ephrin family. Ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. Ephrin B3 is important in brain development as well as in its maintenance. It is especially important for forebrain function since its expression levels were particularly high in several forebrain subregions compared to other brain subregions. Ephrin B3 binds to, and induce the collapse of, commissural axons/growth cones in vitro. It may play a role in constraining the orientation of longitudinally projecting axons.

Ephrin B3 (Predicted)

EFL-6; ELK-L3; EPH-RELATED RECEPTOR TRANSMEMBRANE LIGAND ELK-L3; LERK-8; RP23-56I20.5; ELF-3; EPL8; EPHRIN B3 / EFNB3; EPLG8; EPHRIN-B3; NLERK-2; EFL6; LERK8; EPH-RELATED RECEPTOR TYROSINE KINASE LIGAND 8

G3V7D4

Efnb3; Efnb3_predicted; rCG_32839

Human Cells

A DNA sequence encoding the rat EFNB3 (G3V7D4) (Met1-Ser224) was expressed, fused with a polyhistidine tag at the C-terminus.

Rat

The recombinant rat EFNB3 comprises 208 amino acids and predicts a molecular mass of 23.1 kDa. The apparent molecular mass of the recombinant protein is approximately 32 kDa in SDS-PAGE under reducing conditions due to glycosylation.

> 85 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA.
Immobilized rat EFNB3-His at 10 μg/ml (100 μl/well) can bind biotinylated mouse EphB3-His (Cat:500494), The EC₅₀ of biotinylated mouse EphB3-His (Cat:500494) is 17.4-40.6 ng/ml.